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好sf13好sf13Anti-APRIL/TNFSF13抗体

作者:ao0 日期:2018-11-25 分类:升级指南

好sf13好sf13

Anti-APRIL/TNFSF13抗体

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

background:

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.

Function:

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.

Subunit:

Homotrimer (Potential).

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.

Post-translational modifications:

The precursor is cleaved by furin.

Similarity:

Belongs to the tumor necrosis factor family.

SWISS:

O75888

Gene ID:

8741

Database links:

Entrez Gene: 8741Human

Entrez Gene: 69583Mouse

Entrez Gene: 287437Rat

Omim: 604472Human

SwissProt: O75888Human

SwissProt: Q9D777Mouse

Unigene: 54673Human

Unigene: 8983Mouse

Unigene: 19955Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Blank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min)).

Primary Antibody:Rabbit Anti-APRIL/TNFSF13 antibody(bs-5725R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;

Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );

结果表明:在抗体包被浓度为1μg/ml,封闭液为0.25%酪蛋白,洗液为0.5‰pbst,标准品稀释液为pbs,检测抗体浓度为0.5μg /ml时即可良好检测标准品及血浆样本中的masp2,在此条件下建立的标准曲线拟合度、可重复性均较好,检测范围在10-1500ng/ml,符合临床 检测的需要。

background:-weblit-gradient(radial,50 50,50,50 50,0,form(black),color-stop(0.5,red),to(blue))。

dj0.50.6dmax0.50.6400200240mm,取dj240,nj31.5r/min。

2)肥田土50%,圈肥40%,炉灰10%,每立方米加1.5公斤磷酸二铵,0.5公斤硫酸钾,200克多菌灵。

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